This project aims to improve gene manipulation technologies, develop more efficient methodologies for cell transfection, and improve vectors' construction linked to genetically modified pigs and poultry production. These objectives are in synergy with the Genetic Engineering in Agribusiness portfolio and meet demands for genetic engineering innovations on animal species of economic and social interest. Transgenic animals are bred for various purposes and can be used as bioreactors, models for human disease studies, and livestock purposes. Pigs and poultry have been genetically modified for the most diverse applications. Pigs are suitable for transgenic technology because they reach maturity quickly and are highly prolific. Moreover, biomedicine has a great interest in studying pigs due to their similarity in size, physiology, organ metabolism, and gene sequence with humans. In turn, poultry has great importance as a bioreactor. The large number of eggs produced per year and the large amount of protein present in the egg white encoded by a single gene make them suitable for recombinant protein production. Sperm-mediated DNA transfer (SMGT), DNA microinjection into zygote pro-nuclei (PMI), somatic cell nuclear transfer (SCNT), and injection of previously modified somatic/embryonic cells are some of the methodologies for AGM production.