Cattle tick parasitism causes losses of three billion dollars a year in Brazil. The main factor behind this is errors made in combating ticks, leading to the selection and proliferation of populations resistant to commercial acaricides. In addition to this problem, there is a demand for sustainable control strategies. Biological control using entomopathogenic nematodes (EPNs), a line of research conducted at Embrapa Dairy Cattle, represents a promising alternative. Recent research has developed a formulation capable of protecting EPNs from the deleterious effects of the environment and guaranteeing some action on ticks in infested cattle. In an attempt to increase efficiency, the aim of this study was to evaluate the compatibility, survival and efficacy of EPN in a protective formulation associated with plant extracts on the cattle tick. The research, consisting of two experiments and carried out in partnership with UFJF (Federal University of Juiz de Fora) and UFG (Federal University of Goiás), is aligned with UNO (United Nations Organization) Sustainable Development Goal 2 - SDG 2 “Eradicate hunger: End hunger, achieve food security and improved nutrition and promote sustainable agriculture”, especially target 2.4. “By 2030, ensure sustainable food production systems and implement resilient agricultural practices that increase productivity and production, help maintain ecosystems, strengthen the capacity to adapt to climate change, extreme weather conditions, droughts, floods and other disasters, and progressively improve land and soil quality,” as it is based on the search for a solution to one of the biggest health problems in the dairy and meat production chains. In the first experiment, formulated EPNs were added to rosemary essential oil (EO) in concentrations of 5, 10 and 15%, and survival was assessed daily. The combination that resulted in the highest nematode survival rate (EPNs in formulation + rosemary EO 5%, 408 h) was used in the evaluation of cattle tick by spraying adults, resulting in 98.78% efficacy, significantly higher than the group with no combination (81.83%). Compatibility was proven between the nematode in the protective formulation and 5% rosemary essential oil, with positive repercussions on the survival of the biological controller and in vitro efficacy against cattle tick. Although there is a need for further studies, the contributions of this research represent a motivating scenario, signaling the promising use of forms of control that combine efficiency, cost reduction, health preservation, the strengthening of food production chains and environmental sustainability, with positive repercussions for society as a whole.