Author(s): CASTELIANI, A. G. B.; KAVAMURA, V. N.; ZUCCHI, T. D.; SÁBER, M. L.; NASCIMENTO, R. dos S.; FRIGHETTO, R. T. S.; TAKETANI, R. G.; MELO, I. S. de

Summary: Abstract: The increase on the worldwide influx of solar ultraviolet radiation (UV-B) has inflicted a considerable challenge, due to its deleterious effects to live beings and pose a special threat to phyllosphere communities. However, UV-B influence on epiphytic yeasts associated with agricultural crops remains limited. Main aim of the present study was to determine the effect of ultraviolet-B radiation on the epiphytic yeast populations associated with strawberry under field conditions. Thus, strawberries (Fragaria x ananassa Duchesne cv. Oso Grande) were grown under three different treatments: a) environmental UV-B, b) enhanced UV-B and c) decreased UV-B; thereafter, their yeast epiphytic populations were analyzed by T-RFLP prior to yeast isolation, identification and in vitro test for the sensitivity against UV-B. Our results demonstrated that UV-B radiation did not significantly affect the strawberry epiphytic yeast populations. However, isolates directly exposed to radiation, generally revealed morphological abnormalities and a diminishing value in the percentage of survival, although they remained constant after 240 min of exposure. The increase in UV-B radiation was not sufficient to affect the dynamics and composition of epiphytic yeast communities from strawberry, there was a clear morphotype shift towards the selection of pigmented isolates.

Publication year: 2014

Types of publication: Journal article

Unit: Embrapa Environment

Keywords: Clima, Field conditions, Global warming, Leaf surface, Levedo, Morango, Phyllosphere, Raio ultravioleta, Strawberries, T-RFLP, Ultraviolet radiation, Yeasts