Expression of LH receptor isoforms during follicle deviation in cattle.
Expression of LH receptor isoforms during follicle deviation in cattle.
Author(s): WOHLRES-VIANA, S.; ARASHIRO, E. K. N.; CAMARGO, L. S. de A.; MACHADO, M. A.; FERNANDES, C. A. C.; VIANA, J. H. M.
Summary: The aim of this study was to evaluate the changes in the expression of LHR isoforms associated with follicle divergence in cattle, using dairy breeds with different dominant follicle sizes at deviation as models. Granulosa cells (GC) were collected using an adapted ultrasound-guided follicular aspiration system (Arashiro et al., Reprod Biol Endocrinol, 11:73, 2013) from follicles of 6, 8, 10 or 12 mm in diameter of Holstein, and of 4, 6, 8 or 10 mm of Gir heifers. The recovered follicular fluid was centrifuged and the cells were washed with NaCl 0.9% saline and kept in RNA Later (Ambion).
Publication year: 2014
Types of publication: Abstract in annals or event proceedings
Unit: Embrapa Dairy Cattle
Keywords: Cópia, LHR, Splicing alternativo
Observation
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