Effect of lentiviral vectors microinjection into the perivitelline space of bovine early zygotes on embryo development and transgene expression.
Effect of lentiviral vectors microinjection into the perivitelline space of bovine early zygotes on embryo development and transgene expression.
Author(s): CAMARGO, L. S. de A.; OTERO, R. J.; GONÇALVES, F. S.; MUNK, M. P.; SOUZA, E. D.; IGUMA, L. T.; TOLEDO, J. R.; PARRA PEREIRA, N. C.; QUINTAO, C. C. R.; VIANA, J. H. M.
Summary: The present study evaluated embryo development and transgene expression after lentiviral microinjection into perivitelline space of early zygotes (6 h post in vitro fertilization) or matured oocytes. In a preliminary experiment we evaluated the effect of short incubation period and of sperm concentration on embryo development. In vitro matured oocytes (n = 591, five repetitions) were co-incubated with 2 or 4 million sperm/mL for 6 or 20 h and cleavage and blastocyst rate were analyzed by Chi square. Higher (P\0.05) cleavage rate was obtained with 4 million sperm for 20 h whereas the blastocyst rate at day seven post-insemination was similar (P\0.05) between 4 million sperm for 6 and 20 h.
Publication year: 2014
Types of publication: Abstract in annals or event proceedings
Unit: Embrapa Dairy Cattle
Keywords: Blastocitos, Oocitos, Zigotos
Observation
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