Autoria: NÓBREGA, I. C. C.; PEREIRA, G. E.; SILVA, M.; PEREIRA, E. V. S.; MEDEIROS, M. M.; TELLES, D. L.; ALBUQUERQUE JUNIOR, E. C.; OLIVEIRA, J. B.; LACHENMEIER, D. W.

Resumo: An improved sample preparation procedure for analysis of carcinogenic ethyl carbamate (EC) in wine by GC?MS?SIM is proposed. Differences over AOAC reference procedure were: (1) use of EC-d5 as internal standard instead of less similar propyl carbamate; (2) extraction by diethyl ether instead of more toxic dichloromethane, and (3) concentration by vacuum automated parallel evaporation instead of more time and work consuming rotary evaporation. Mean recovery was 104.4%, intraday precision was 6.7% (3.4gL1) and 1.7% (88.5lgL1), regression coefficient was 0.999 in the linear working range of 3?89lgL 1, and limits of detection and quantification were 0.4 and 1.2 lgL 1. Applicability was demon- strated by analysis (in triplicate) of 5 wine samples. EC concentration ranged from 5.2 ± 0.2 to 29.4 ± 1.5lgL1. The analytical method is selective, accurate, repeatable, linear, and has similar method performance as the reference method along with the several mentioned advantages.

Ano de publicação: 2015

Tipo de publicação: Artigo de periódico

Unidade: Embrapa Uva e Vinho

Palavras-chave: Automated parallel evaporation, Diethyl ether extraction, Ethyl carbamate, Etil carbamato, Evaporação paralelo automatizado, GC–MS–SIM, Método analítico, Vinho, Wine