Autoria: STEINMETZ, R. L. R.; MEZZARI, M. P.; SILVA, M. L. B. da; KUNZ, A.; AMARAL, A. C.; TÁPPARO, D. C.; SOARES, H. M.

Resumo: Appropriate enrichment of anaerobic microorganism?s consortium is crucial for accurate biochemical methane potential (BMP) assays. An alternative method to produce and maintain a mesophilic methanogenic inoculum was demonstrated. Three sources of inoculum were mixed and acclimated for 857 days in order to reach steady conditions (pH = 7.90 ± 0.46; VS/TS > 50%; VFA/alkalinity = 0.16 ± 0.04 gAcetic Acid/gCaCO3 ). Biogas yield >80% was obtained after 70 days of inoculum acclimation in comparison to standard cellulose (>600 mLN/gVS). Methanogen community analysis based on 16S rDNA of the inoculum revealed Archaea concentration of 3 1012 gene copies/g (Methanobacteriales 8 1010; Methanomicrobiales 8 1010; and Methanosarcinales 4 1011 gene copies/g). The proposed method for development and maintenance of microorganism enrichment inoculum demonstrates consistent BMP data which is a requirement for dependable prediction of biogas production at field scale operations.

Ano de publicação: 2016

Tipo de publicação: Artigo de periódico

Unidade: Embrapa Suínos e Aves

Palavras-chave: Anaerobic digestion, Biogás, Dejeto, Digestão anaeróbica, Waste treatment