Influence of Mini-Percoll techniques on motility parameters of ram frozen-thawed sperm.

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Author(s): OLIVARES, C.; FONSECA, J. F. da; SARAIVA, H.; ALFRADIQUE, V.; CÔRTES, L.; COSTA, L.; BRANDÃO, F.; SOUZA-FABJAN, J. M.

Summary: The aim of this study was to compare the influence of different forces and time of centrifugation in mini-Percoll techniques with traditional Percoll for sperm selection on motility parameters of ram frozen-thawed sperm. For the first time, we have demonstrated that the reduction of the gradient volume and time of centrifugation, and the increase of its force at Percoll, can be successfully used for ram sperm selection. In conclusion, mini-Percoll may be used instead of traditional Percoll, decreasing costs and time of sperm handling, without motility damages in ram frozen-thawed sperm.

Publication year: 2016

Types of publication: Abstract in annals or event proceedings

Unit: Embrapa Goats & Sheep

Keywords: Carneiro, Cinética espermática, Espermatozóide, Força e tempo de centrifugação, Gradiente de Percoll, Motilidade espermática, Movemen, Ovino, Rams, Reprodução animal, Seleção espermática, Sexagem, Sexual reproduction, Sheep, Sperm quality, Spermatozoa, Sêmen

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