Production of cellulolytic and hemicellulolytic enzymes by Aspergillus niger 3T5B8 mutants and parental strains in solid-state fermentation.
Production of cellulolytic and hemicellulolytic enzymes by Aspergillus niger 3T5B8 mutants and parental strains in solid-state fermentation.
Author(s): GOTTSCHALK, L. M. F.; TERZI, S. da C.; SOUZA, E. F. de; OLIVEIRA, E. M. M.; FAVARO, L. C. de L.; PENHA, E. das M.
Summary: Several studies have sought to increase the efficiency of the enzymatic hydrolysis of lignocellulosic biomass. In this context, the improvement of strains producing cellulolytic and hemicellulolytic enzymes may result in more efficient enzymatic mixtures. The parent strain of Aspergillus niger 3T5B8, referred as a polygalacturonase producer, was used for genetic improvement in order to increase the production of cellulases and hemicellulases. The production of the enzymes CMCase, xylanase, beta-glucosidase and polygalacturonase by solid-state fermentation using two mutant strains P49 and P83 was evaluated and compared with the parental strain. The results showed a prominence for the P83 strain with an increase in the production of 174% for xylanase and 402% for polygalacturonase.
Publication year: 2017
Types of publication: Paper in annals and proceedings
Unit: Embrapa Food Technology
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