Author(s): GOMES COPELAND, K. K. P.; LEDO, A. da S.; DAVID, J. P.; ARAUJO, A. G. de; ALMEIDA, F. T. C. de

Summary: this work describes the establishment of procedures to induce in vitro callogenesis from Poincianellapyramidalis (Tul.) L. P. Queiroz, Fabaceae, explants. Nodal, internodal and leaf segments were iso-lated from in vitro germinated seedlings and cultured in MS medium with 0, 2.5, 5 and 10 mg l−1of2,4-dichlorophenoxyacetic acid. After 30 days, the explants with induced callus showed a quadraticresponse for the segments nodal, internodal and leaf, with increasing the callus formation in 2,4-dichlorophenoxyacetic acid concentrations of 6.28, 6.49 and 4.91 mg l−1, respectively. In 30 days there wasa linear oxidation rise with the increase to the 2,4-dichlorophenoxyacetic acid. After 60 days, oxidationvalues were minimum, at 2,4-dichlorophenoxyacetic acid concentrations of 5.13 mg l−1(internodal) and3.98 mg l−1(leaf). The highest callus production was observed after 30 days in the presence of 6.09 mg l−1,5.82 mg l−1and 4.91 mg l−1of 2,4-dichlorophenoxyacetic acid in nodal, internodal and leaf segments,respectively. After 60 days these segments showed peaks of production at 7.0 mg l−1(nodal), 6.15 mg l−1(internodal) and 5.08 mg l−1(leaf) of 2,4-dichlorophenoxyacetic acid. For callus induction the intake of2,4-dichlorophenoxyacetic acid was essential. The greater intensity in callus formation was observed in4.91 mg l−1in leaf segments after 30 days.

Publication year: 2017

Types of publication: Journal article

Unit: Embrapa Coastal Tablelands

Keywords: Callogenesis, Genética de planta