Author(s): SCHMIDT, M. E. P.; PIRES, F. B.; BRESSAN, L. P.; SILVA JUNIOR, F. V. da; LAMEIRA, O. A.; ROSA, M. B. da

Summary: The aim of this paper is to provide an overview on the chemical composition of triterpenes in widespreadused folk medicine species, through the development and validation of eleven compounds using HPLC-UV detection. The compounds were separated using isocratic elution, on a reverse phase column (KinetexC18, 250 mm × 4.6 mm, 5 m) with mobile phase consisted of acetonitrile:tetrahydrofuran (90:10, v/v),flow-rate of 0.5 ml/min and detection in 210 nm. Diverse validation parameters were successfully eval-uated. The samples of Bauhinia variegata L., B. variegata var. candida Voigt, Fabaceae, Cecropia palmataWilld. and C. obtusa Trécul, Urticaceae, collected in 2012, 2013 and 2014 from Amazon were treated withtwo different solvents (ethyl acetate and chloroform) and analyzed by the proposed method. Stigmas-terol, lupeol, -sitosterol, -amirin and -amirin were found in all the studied plants. Highlighting thepresence of oleanolic acid, maslinic acid in C. obtusa and C. palmata extracts, erythrodiol only in C. pal-mata, stigmasteol in B. variegata and -amirin in B. variegata var. candida. Overall, ethyl acetate showedbetter performance as the extractor solvent than chloroform. Moreover, it could be used for the qualitycontrol of medicinal plants and to assess potential marker compounds.

Publication year: 2018

Types of publication: Journal article

Unit: Embrapa Eastern Amazon

Keywords: Composição Química, Planta Medicinal