Autoria: FERRÃO, L. F. V.; CAIXETA, E. T.; SOUZA, F. de F.; ZAMBOLIM, E. M.; CRUZ, C. D.; ZABOLIM, L.; SAKIYAMA, N. S.

Resumo: The genetic variability characterization of the accessions of the germplasm collection, using molecular markers, is being applied as a complementary strategy to the traditional approaches to redefine the plant genetic resources. In this study, we compared the informativeness and efficiency of the molecular markers RAPD, AFLP and SSR in the analysis of 94 accessions of Coffea canephora germplasm held by the breeding program of the Brazilian Agricultural Research Corporation (Embrapa), Rondonia State, Brazil. For this, we considered the marker?s discriminatory power and level of polymorphism detected and also the genetic relationships and clustering (dendrogram) analysis. The RAPD marker yielded low-quality data and problems in the discrimination of some accessions, being less recommended for genetic studies of C. canephora . The SSRs had a higher level of information content and yielded high-quality data, while AFLP was the most efficient marker system because of the simultaneous detection of abundant polymorphism markers per few reactions. Our results indicate that AFLP and SSR, allies to the intrinsic characteristics of each technique, are the most suitable molecular markers for genetic studies of C. canephora .However, the choice of AFLP or SSR in the species characterization should be made in agreement with some characteristics that are discussed in this work.

Ano de publicação: 2013

Tipo de publicação: Artigo de periódico

Unidade: Embrapa Semiárido

Palavras-chave: AFLP, Café, Coffee, Melhoramento genético, RAPD, Robusta coffee, SSR, genetic relationships