Author(s): CORPES, R.; MONTEIRO, L.; ANDRADE, E. H.; MAIA, J. G.; MENEZES, I. C. de; SETZER, W. N.; RAMOS, A. R.; SILVA, J. K. R. da

Summary: In this study, P. divaricatum (Piperaceae) plants were subcultured by cuttings and cell tissue in order to carry out a comparison of the volatile profile, phenolic compounds, and antioxidant activity. Propagation by cuttings used vermiculite substrate and Murashige-Skoog (MS) medium in the absence of growth regulators and rooting started at 15 days of growth providing a higher number of plants after 90 days. In vitro propagation was performed using shoot apices as explants in MS media supplemented with 0.5 mg mL-1 BA (6-benzyladenine). Volatile profiles analyzed by gas chromatography-mass spectrometry (GC-MS) showed as main compounds methyl eugenol, E-?-ocimene, and ?-elemene in the in vivo and in vitro cultures. Phenolic contents determined by the Folin-Ciocalteu method had no significant difference at the end of 90 days of growth displaying a good linear correlation with antioxidant activity and phenylpropanoids amounts (r > 0.7). However, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging was higher for in vitro plants grown in comparison to in vivo plants cultured.

Publication year: 2019

Types of publication: Journal article

Unit: Embrapa Eastern Amazon

Keywords: Antioxidante, Micropropagação, Piperaceae, Propagação Vegetativa