Separation of antbacterial biocompounds from Hancorni speciosa leaves by a sequential process of pressurized liquid extraction.
Separation of antbacterial biocompounds from Hancorni speciosa leaves by a sequential process of pressurized liquid extraction.
Author(s): BARBOSA, A. M.; SANTOS, K. S.; BORGES, G. R.; MUNIZ, A. V. C. da S.; MENDONCA, F. M. R.; PINHEIRO, M. S.; FRANCESCHI, E.; DARIVA, C.; PADILHA, F. F.
Summary: Hancornia speciosahas bioactive molecules such as phenolic compounds which exhibit antioxidant and anti-microbial properties. Nevertheless, the separation of these substances from a medicinal plant can be differentaccording to the extraction process and solvent used. Thus, the present study aimed was to separate phenoliccompounds fromHancornia speciosaleaves in three steps by using a sequential pressurized liquid extraction andevaluated their antioxidant and antibacterial activity. Results showed that the temperature increase from 25 to60° C provides a significative enhancement in the extraction yield. The raw ethanol extract (39.0 ± 0.2%)showed the highest overall extraction yield. However, fractionated ethanol extract (FEE) at 60 °C exhibited thehighest amount of total phenolic compounds (347.0 ± 4.12 mg/g extract). Fractionated ethyl acetate extract(FAE) at 60 °C presented improved quantity of totalflavonoids (230.4 ± 3.5 mg/g extract) and rutin content(16.6 ± 0.3 mg/mL extract). The antioxidant activity results for IC50of DPPH indicated that FEE(3.8 ± 0.1 ?g/mL) was the best free radical scavenger. FAE and FEE were able to inhibit the growth of mul-tidrug-resistantE. coli.
Publication year: 2019
Types of publication: Journal article
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