Autoria: PRADO, G. S.; PINHEIRO, T. T.; FARIA, J. C. de; VIANELLO, R. P.

Resumo: We will focus here on the use of the CRISPR/Cas system to induce mutations via NHEJ. The NHEJ mechanism acts in most of the cell cycle and is not based on homology to repair DNA, causing mutations such as insertions or deletions (indels) or even substitutions. In this way, the expression of the genes in the place where the repair occurred by NHEJ can, in general, be interrupted, and the encoded proteins may not be functional. To clarify the process of genome editing by CRISPR, this chapter aims to address the details of the NHEJ mechanism, as well as the use of ribonucleoprotein systems (RNPs) to promote editing, in addition to providing a practical procedure for the design of experiments.

Ano de publicação: 2021

Tipo de publicação: Parte de livro (capítulos de livros, trabalhos e resumos publicados em anais ou em coletâneas)

Unidade: Embrapa Arroz e Feijão

Palavras-chave: Biotechnology, Biotecnologia, CRISPR, CRISPRevolution, DNA, Engenharia Genética, Genetic engineering, Genoma, Non-homologous, Nucleases, Plant genetics, RNA, RNA editing, Ribonucleoproteins