Modulation of gene expression in plants via CRISPR/dCas9 technology.

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Author(s): MORGANTE, C. V.; ARRAES, F. B. M.; MOREIRA-PINTO, C. E; MELO, B. P.; SA, M. F. G. de

Summary: This chapter discusses CRISPR technology using the dead Cas9 variant (CRISPR/ dCas9), emphasizing on its use for modulating gene expression in plants. The main strategies currently used are presented and discussed, for which a script was proposed, considering the main aspects for a good experimental design.

Publication year: 2021

Types of publication: Book sections

Unit: Embrapa Semi-arid Region

Keywords: Agricultural biotechnology, Biotecnologia, CRISPR, CRISPR Cas, CRISPR technology, DNA, Expressão gênica, Genetic engineering, Genoma, Genoma da planta, Melhoramento Genético Vegetal, Sistema dCas9

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