Development of an enzyme-coated microcantilever-based biosensor for specific detection of short-chain alcohols.
Development of an enzyme-coated microcantilever-based biosensor for specific detection of short-chain alcohols.
Author(s): MARGARIDO, A.; MANZINE, L. R.; ARAUJO-MOREIRA, F. M.; GONÇALVES, R. V.; HERRMANN JUNIOR, P. S. de P.
Summary: This paper describes the development of a biosensor designed for the enzymatic detection of short-chain alcohols. The biorecognition element, alcohol dehydrogenase, was immobilized on selfassembled monolayers deposited on top of silicon nitride microcantilevers. The self-assembly process was performed by surface activation using 3-aminopropyltriethoxysilane, followed by glutaraldehyde and biomolecule binding. X-ray photoelectron spectroscopy and atomic force microscopy were used. The biosensor showed a lower response time and sensibility from 0.03 to 1.2 mL/L. Its selectivity was analyzed through exposure to pure and mixed volatile solvents. Sensor sensibility was higher in the presence of short-chain alcohols and practically null involving other polar or nonpolar solvents.
Publication year: 2021
Types of publication: Journal article
Unit: Embrapa Instrumentation
Keywords: Biorecognition element, Biosensor, Microcantilever
Observation
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