Author(s): ANGELO, P. C. da S.; ARIYOSHI, C.; CAIXETA, E. T.; PEREIRA, L. F.

Summary: RATIONALE: The SH3 locus is implicated in plant defense against the leaf rust disease caused by the fungi Hemileia vastatrix, which reduces coffee plant production. A cluster of genes coding for NB-LRR proteins is allocated to this locus. Investigating the regulatory region of' these genes can be worthy in knowledge and economically. METHODS: The cis-acting elements (cis-els, conserved motifs for the interaction with transcription machinery) in the promoters (2000 pb up-stream translation start) of 20 genes allocated to paralogous SH3 loci in four coffea spp. genotypes were identified and counted (http://www.dna.affrc.go.jp/place). Data were analyzed using Sigma Plot. RESULTS: Cis-els more frequently identified are depicted in Fig. 1 (in average, 3.5 times/promoter for SEF4MOTIFGM7S up to 14.1 times for CAATBOXl), Genes diverged for the frequency of cis-els. At least one gene/genotype was found in the highest cis-el frequency class (11.46 < N3, A2, Cl, B8 > 11.9 copies of each cis-el), probably displaying complex patters of induction. Genotypes did not diverge. CONCLUSIONS & PERSPECTIVES: Information presented here are going to be correlated with the transcription level of different genes.

Publication year: 2021

Types of publication: Abstract in annals or event proceedings

Unit: Embrapa Coffee

Keywords: Coffea, Genotype, Genética Vegetal, Species diversity