Autoria: PAIXÃO, R. V.; SILVA, G. F. da; CAETANO, A. R.; CINTRA, L. C.; VARELA, E. S.; O'SULLIVAN, F. L. A.

Resumo: The genes coding for Cytochrome P450 aromatase (cyp19a1a and cyp19a1b) and estrogen (E2) receptors (esr1, esr2a and esr2b) play a conserved role in ovarian differentiation and development among teleosts. Classically, the "gonad form" of aromatase, coded by the cyp19a1a, is responsible for the ovarian differentiation in genetic females via ligation and activation of the Esr, which mediates the endocrine and exocrine signaling to allow or block the establishment of the feminine phenotype. However, in neotropical species, studies on the molecular and endocrine processes involved in gonad differentiation as well as on the effects of sex modulators are recent and scarce. In this study, we combined in silico analysis, real-time quantitative PCR (qPCR) assay and quantification of E2 plasma levels of differentiating tambaqui (Colossoma macropomum) to unveil the roles of the paralogs cypa19a1a and cyp19a1b during sex differentiation.

Ano de publicação: 2022

Tipo de publicação: Artigo de periódico

Unidade: Embrapa Amazônia Ocidental

Palavras-chave: Aromatase, Colossoma Macropomum, Cyp19a1, Neotropical fish, Promoter Structure, Sex development, Synteny, Tambaqui