Analysis of fungal co-culture metabolites using both classic and imprinting Mass Spectrometry Imaging.
Analysis of fungal co-culture metabolites using both classic and imprinting Mass Spectrometry Imaging.
Author(s): TEIXEIRA, T. S.; RODRIGUES NETO, J. C.; CONCEIÇÃO, A. A.; COSTA, P. P. K. G.; SIQUEIRA, F. G. de; ABDELNUR, P. V.
Summary: Fungi are able to produce a wide range of bioactive metabolites, which enhanced when challenged in co-culture[1]. For a better evaluation of these metabolites, the Mass Spectrometry Imaging (MSI) can be used to provide complementary information through the metabolite spatial localization [2]. However, some adaptations are required on available methodologies in MSI for applications in microrganisms[2], particularly on sample preparation. The imprinting method has been shown to be a robust method when applied to preparation of samples analyzed by DESI-MSI[3], but it has never been tested for MALDI-MSI for microrganisms, in our knowledge.
Publication year: 2022
Types of publication: Abstract in annals or event proceedings
Unit: Embrapa Agroenergy
Keywords: Aspergillus terreus, MALDI-MSI, Metabolomics, Pleorotus pulmonarius
Observation
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