Analysis of virome by high-throughput sequencing disclosed multiple infection in a single grapevine plant.
Analysis of virome by high-throughput sequencing disclosed multiple infection in a single grapevine plant.
Author(s): FAJARDO, T. V. M.; NHANI JUNIOR, A.; NICKEL, O.
Summary: Among phytosanitary problems of the grapevine, viruses stand out for their capacity of reducing the quality and yield of grapes. however, detecting and identifying viral infections in grapevines can be challenging. This study performed a high throughput sequencing (hTS) of the viral pathogens present in a vine showing virus-like symptoms to elucidate the etiology. hTS analysis reported in a hybrid grapevine with mild curling down of leaf edges, the presence of four viruses and viroids, which were probably implicated in the observed symptoms. The determined complete genomes showed high genetic identities with previously characterized isolates of homologous pathogens. Key words: diagnosis, symptoms, hTS, diversity, virus. Resumo: Dentre os problemas fitossanitários da videira, os vírus se destacam pela capacidade de reduzir a qualidade e o rendimento da uva. No entanto, detectar e identificar infecções virais em videiras pode ser um desafio. O objetivo do estudo foi realizar um sequenciamento de alto rendimento (hTS) para determinar os patógenos virais presentes em uma videira com sintomas de virose e elucidar a etiologia. com o hTS foi detectada, em uma videira híbrida com leve enrolamento dos bordos foliares, a presença de quatro vírus e viroides, os quais provavelmente estavam implicados com os sintomas observados. Os genomas completos determinados mostraram altas identidades genéticas com isolados previamente caracterizados de patógenos homólogos. Palavras-chave: diagnose, sintomas, hTS, diversidade, vírus.
Publication year: 2023
Types of publication: Journal article
Unit: Embrapa Grape & Wine
Keywords: Diagnose, Diagnosis, Diversidade, Diversity, HTS, Sequenciamento de alto rendimento, Sintomas, Symptoms, Videira, Vírus
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