Enabling genome editing in tropical maize lines through an improved, morphogenic regulator-assisted transformation protocol.

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Author(s): HERNANDES-LOPES, J.; PINTO, M. S.; VIEIRA, L. R.; MONTEIRO, P. B.; GERASIMOVA, S. V.; NONATO, J. V. A.; BRUNO, M. H. F.; VIKHOREV, A.; FERNANDES, F. R.; GERHARDT, I. R.; PAUWELS, L.; ARRUDA, P.; DANTE, R. A.; YASSITEPE, J. E. de C. T.

Summary: Here, we report the successful GE of agronomically relevant tropical maize lines using a MR-based, Agrobacterium-mediated transformation protocol previously optimized for the B104 temperate inbred line.

Publication year: 2023

Types of publication: Journal article

Unit: Embrapa Digital Agriculture

Keywords: Agrobacterium, Agrobactéria, B104, BABY BOOM, CRISPR/Cas9, Edição de genoma, Milho, Off-target, Protoplast, Protoplasto, VIRESCENT YELLOW-LIKE, WUSCHEL, Zea Mays

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