Author(s): ARAKAKI, J. E.; VIGNA, B. B. Z.; PESSOA FILHO, M. A. C. de P.; MUDADU, M. de A.; RIOS, E.; FAVERO, A. P.

Summary: The development of new, cost-effective sequencing technologies, along with user-friendly computational tools, facilitates genomic studies of non-model species. Paspalum notatum is an important forage and turfgrass worldwide. Pensacola is a diploid (2n=20) cultivar with haploid genome size of 600Mb, estimated by flow cytometry. Pensacola is native to Argentina and has long narrow leaves, early flowering and cold tolerance. It is related to five ('Tiphi1', 'Tiphi 2', 'UF-Riata', 'Tifton 9' and 'Tifquick') of the 17 released cultivars and the most adopted cultivar in Southeastern Florida and South of Brazil, indicating its agro-economic importance. Here, we present a chromosome-scale genome assembly of Paspalum notatum cv. Pensacola. To obtain the PacBio HiFi sequences, Pensacola seeds were germinated and grown in a greenhouse at the University of Florida, and high molecular weight DNA extraction was performed from leaf tissue. PacBio HiFi sequencing was performed in a Sequel II system at Maryland Genomics, using one SMRT cell 8M in Circular Consensus Sequencing mode (CCS) with 30h of sequencing time.

Publication year: 2023

Types of publication: Abstract in annals or event proceedings

Unit: Embrapa Cerrados

Keywords: Capim Pensacola, Genome, Gramínea Forrageira, Paspalum Notatum