Author(s): NUNES, L. S.; FREIRE, Í. A.; RODRIGUES, K. B.; MIDORIKAWA, G. E. O.; QUECINE, M. C.; FAVARO, L. C. de L.

Summary: We evaluated in vitro different Bacillus, Paenibacillus, Peribacillus, and Alkalihalobacillus strains for their ability to solubilize insoluble inorganic phosphate sources [Ca3(PO4)2; AlPO4; FePO4]. A total of 217 endophytic, epiphytic, and rhizospheric strains isolated from native and cultivated plant species from different Brazilian biomes were obtained from the “Collection of Microorganisms and Microalgae Applied to Agroenergy and Biorefineries (CMMAABio)”. The B. pumilus BRM033255 strain was used as positive control. All 217 bacteria were inoculated (quadruplicate) in solid NBRIP-Ca and NBRIP-Al culture media and incubated at 28oC for 15 days. The data on colony diameters and solubilization halos were collected, and the solubilization index (SI) value was calculated as the ratio between the diameter of the clear zone and the colony. For tests in liquid media, 187 of the 217 strains were inoculated (one replicate) in 250 mL conical flasks containing 50 mL of the NBRIP-Ca, NBRIP-Al, and NBRIP-Fe culture media, with an initial optical density adjusted to 0.2 (OD600nm).

Publication year: 2023

Types of publication: Abstract in annals or event proceedings

Unit: Embrapa Agroenergy

Keywords: Bacillus (bacteria), Bactéria, Bioprospecção, Cana de Açúcar, Fonte inorgânica de fósforo, Fósforo Inorgânico, Inorganic phosphorus, Sugarcane