Production of cellulolytic and hemicellulolytic enzymes by the pectinolytic Aspergillus niger 3T5B8-C88-P83 mutant strain under different cultivation conditions.
Production of cellulolytic and hemicellulolytic enzymes by the pectinolytic Aspergillus niger 3T5B8-C88-P83 mutant strain under different cultivation conditions.
Author(s): CARVALHO, B. A. R.; ALVES, L. M.; MENDES, T. D.; SALUM, T. F. C.; FAVARO, L. C. de L.
Summary: Embrapa Agroenergia has been conducting genetic improvement of filamentous fungi to increase the production of cellulases and hemicellulases to deconstruct lignocellulosic biomass. In a conventional breeding program for Aspergillus niger 3T5B8 performed in 2015, after two rounds of mutagenesis (ultraviolet followed by ethyl methanesulfonate), the pectinolytic mutant strain 3T5B8-C88-P83 was selected, which presents genetic changes in the catabolite repression by glucose pathway and a significant increase in polygalacturonase production compared to the original 3T5B8 strain. This work aimed to evaluate the production of cellulolytic and hemicellulolytic enzymes of the 3T5B8-C88-83 mutant under different cultivation conditions, seeking its future application in the enzymatic processing of cotton fabrics. Endoglucanase production by the 3T5B8-C88-P83 mutant strain was evaluated in 250 mL Erlenmeyer flasks containing different liquid and solid culture media (four repetitions).
Publication year: 2023
Types of publication: Abstract in annals or event proceedings
Unit: Embrapa Agroenergy
Observation
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