Microsatellite markers in maize: challenges and guidelines for implementing multiplex SSR analyses.

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Author(s): KOLTUN, A.; SILVA, P. A. da; TORRES, I. Y.; BONIFÁCIO-ANACLETO, F.; YASSITEPE, J. E. de C. T.

Summary: This study tested two previously constructed simple sequence repeat (SSR) sets containing ten primer pairs each (ten-plex) in 1142 maize genotypes. Here, we describe the challenges faced when implementing this microsatellite-based genotyping protocol in our laboratory and possible ways to overcome them, hopefully aiding other novice research teams in this field.

Publication year: 2024

Types of publication: Journal article

Unit: Embrapa Digital Agriculture

Keywords: Fluorophore interference, Interferência de fluoróforo, Manual allele scoring, Marcadores microssatélites, Milho tropical, Multiplex PCR, Polymerase chain reaction, Pontuação alélica manual, Pull-up, Reação em cadeia da polimerase, Tropical maize

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