Autoria: BARROS, J. R. A.; GUIMARÃES, M. J. M.; SILVA, R. L. de O.; SILVA, J. B. da; BARROS, A. A. G. de; ANGELOTTI, F.; MELO, N. F. de

Resumo: Plants exposed to adverse environmental conditions develop molecular mechanisms of adaptation and/or defense, the osmoprotectors, which function as compatible solutes and contribute to tolerance via prevention systems and protection against cellular damage caused by these abiotic stresses. This study aimed to identify and characterize the osmoprotectors proline and trehalose in cowpea plants cv. Carijó under controlled conditions of water-deficit and heat stress based on the IPCC scenario of 4.8 °C increase in temperature, evaluating their structure and function through computational methods, as well as gene expression by RT-qPCR. The experimental assays were carried out in growth chambers under controlled conditions with different levels of soil water availability, phenological phases and temperature regimes. From the in silico analyses, ten TPS genes and one P5CR gene were identified in Vigna unguiculata, and these were named according to their chromosomal location. The VuP5CR and VuTPS genes play roles in hormone pathway signaling and in the response to light and biotic and abiotic stresses. The genes P5CR (proline) and ?TPS6 (trehalose) were induced with increased temperature and lower water availability in the vegetative phase of cowpeas. In addition, P5CR also showed induction with 50% water availability at high temperatures. In the pod filling phase, the P5CR and ?TPS6 genes were repressed with water availability of 75%, while only the P5CR gene was induced when water availability was reduced to 25% under heat stress. P5CR and TPS6 genes were induced in cowpea cv. Carijó in response to associated abiotic stresses (water-deficit and high temperatures), which suggests their participation in the mechanisms of adaptation of the species in adverse environmental conditions.

Ano de publicação: 2024

Tipo de publicação: Artigo de periódico

Unidade: Embrapa Semiárido

Palavras-chave: Abiotic stress, Agricultura, Climate change, Cultura resistente, Estresse abiótico, Feijão de Corda, Feijão vigna, Mudança Climática, Osmoprotetores, Proline, Resistência a Seca, Stress, Trehalose, Variedade Resistente, Vigna Unguiculata