Author(s): LIMA, M. dos S.; MONTEIRO, L. I. da S.; CARVALHO, A. J. de B.; BASTOS, D. C.; PIMENTEL, T. C.; PIMENTEL, T. C.; MAGNANI, M.

Summary: Reliable analytical methods are the basis for the elucidation of phenolic compounds in foods. This study aimed to optimize and validate a method for determining 42 phenolics using reverse-phase (RP) high-performance liquid chromatography (HPLC) coupled to diode-array-detector-DAD. The performance of two RP columns was eval- uated. The 150x4.6 mm 3-?m column showed superior separation quality, whereas 35 of the 42 phenolics showed a separation resolution ?1.5. The method’s linearity, precision (coefficient variation< 3.09%), recovery (87.5-103.2%), specificity, limits of detection (0.04-0.25 mg/L), and quantification (0.06-0.25 mg/L) had acceptable ranges. Thirty phenolics were quantified in Citrus peels, mainly flavanones, flavanols, flavonols, and phenolic acids, highlighting the high values of hesperidin (535-35070 mg/kg) and naringin (26-36466 mg/kg). Lemon peels named ’Lisboa,’ ’Thaiti,’ ’Thaiti-2000’, and ’Thaiti-2001’ presented the main phenolics associated with antioxidant capacity. The presented method was robust for determining 42 phenolic compounds, offering a new approach for bioactive compound quantification in food matrices.

Publication year: 2024

Types of publication: Journal article

Unit: Embrapa Semi-arid Region

Keywords: Antioxidant activity, Atividade antioxidante, Bioactive compounds, Casca de fruta, Colunas de sílica, Compostos bioativos, Cromatografia líquida, Espécies cítricas, Fruta Cítrica, Liquid chromatography