Author(s): BARBOSA, E. T.; OKURA, M. H.; DIAS NETO, J. J.; BARBOSA, D. B. M.; VIANA, T. A.; LOBO JUNIOR, M.

Summary: Trichoderma is a widely commercially employed filamentous fungus, notable for its utility in various industrial sectors, including pharmaceutical, agronomic, food, energy, and chemical industries. Its capacity to produce a variety of metabolites from different substrates contributes to this usefulness. This study aimed to evaluate the growth and production of secondary metabolites by a promising strain of Trichoderma in different culture media (Sabouraud Agar, Agar-Agar, Czapek Agar, Potato Dextrose Agar, Mannitol Agar, Tryptic Soy Agar, Yeast Extract Peptone Dextrose Agar, Yeast Extract Peptone Agar, and Malt Extract Agar). A disc containing Trichoderma spores and mycelium was inoculated in the center of each plate, with incubation at 25ºC for 5 days. In sequence, the treatments were compared by the diameter of the colonies. After extraction with ethyl acetate, the samples were subjected to HPLC for metabolite quantification. The Trichoderma strain showed better mycelial growth and sporulation in PDA and Malt Extract Agar. However, according to the chromatogram, the bioagent produced metabolites in the 16 - 20 minute interval, best represented by production in the Sabouraud medium. We concluded that the best substrate for metabolite production and identification is the Sabouraud culture medium.

Publication year: 2024

Types of publication: Paper in annals and proceedings

Unit: Embrapa Rice & Beans

Keywords: Antagonismo, Fungal antagonists, Fungo, HPLC, Secondary metabolites, Trichoderma