Influence of nitrogen source on Trichoderma Harzianum culture for cellulolytic enzyme production.
Influence of nitrogen source on Trichoderma Harzianum culture for cellulolytic enzyme production.
Author(s): COSTA, F. K. C.; SOUSA, L. T. M.; MENDES, T. D.; RESENDE, L. S.; COA, F. L. C.; RODRIGUES, C. V. S.; RODRIGUES, D. de S.; SECCHI, A. R.; BON, E. P. S.; GAMBETTA, R.
Summary: The effort to produce cellulases to reduce the overall expenses associated with the second-generation ethanol process holds paramount importance within the biofuel sector. This study aimed to produce cellulases by Trichoderma harzianum using peptone, urea, and ammonium sulfate individually as nitrogen sources in the growth medium for the degradation of pretreated sugarcane bagasse. Statistical analysis of the findings revealed that the highest levels of FPase and ?-glucosidase (0.45 and 0.63 IU/mL, respectively) activity were obtained using the APEP medium, exclusively containing peptone as the nitrogen source. In the enzymatic hydrolysis of pretreated sugar cane bagasse, APEP presented the best glucose yield (5.33 g/L). This is evidence that additional sources of nitrogen are not necessary for favorable results.
Publication year: 2024
Types of publication: Paper in annals and proceedings
Unit: Embrapa Agroenergy
Observation
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