Author(s): SILVA, A. P. M. DA; SILVA, G. S. DA; OIRAM FILHO, F.; SILVA, M. F. S.; ZOCOLO, G. J.; BRITO, E. S. de

Summary: The growing focus on sustainable use of natural resources has brought attention to cashew nut shell liquid (CNSL), a by-product rich in anacardic acids (AAs) with potential applications in diabetes treatment. In this study, three different AAs from CNSL, monoene (15:1, AAn1), diene (15:2, AAn2), and triene (15:3, AAn3), and a mixture of the three (mix) were evaluated as ?-glucosidase inhibitors. The samples were characterized by combining 1D and 2D NMR spectroscopy, along with ESI-MS. In vitro assays revealed that AAn1 had the strongest inhibitory effect (IC50 = 1.78 ± 0.08 ?g mL?1), followed by AAn2 (1.99 ± 0.76 ?g mL?1), AAn3 (3.31 ± 0.03 ?g mL?1), and the mixture (3.72 ± 2.11 ?g mL?1). All AAs significantly outperformed acarbose (IC50 = 169.3 ?g mL?1). In silico docking suggested that polar groups on the aromatic ring are key for enzyme–ligand binding. The double bond at C15, while not essential, enhanced the inhibitory effects. Toxicity predictions classified AAs as category IV, and pharmacokinetic analysis suggested moderately favorable drug-like properties. These findings highlight AAs as a promising option in the search for new hypoglycemic compounds.

Publication year: 2024

Types of publication: Journal article

Unit: Embrapa Foods and Territories

Keywords: Alfa-glucosidase inhibition, Alkyl phenol, Anacardic acid, Antidiabetic compounds, By-product, Drug likeness, Molecular docking, Pharmacokinetic properties