Author(s): CUEVA-YESQUÉN, L. G.; SARTORATTO, A.; SANTOS, A. da S.; MELO, I. S. de; FANTINATTI-GARBOGGINI, F.

Summary: Abstract: A polyphasic approach was applied to characterize taxonomically a novel endophytic bacterial strain, designated as EP178T, which was previously isolated from Passiflora incarnata leaves and characterized as plant-growth promoter. The strain EP178T forms Gram stain-negative and rod-shaped cells, and circular and yellow-pigmented colonies. Its growth occurs at 10–37 °C, at pH 6.0–8.0, and tolerates up to 7% (w/v) NaCl. The major cellular fatty acids found were summed feature 8 (C18:1 ?7c), summed feature 3 (C16:1 ?6c /C16:1 ?7c), and C16:0, and the predominant ubiquinone was Q-9. The phylogenetic and nucleotide-similarity analysis with 16S rRNA gene sequences showed that strain EP178T belongs to Pseudomonas genus. The genomic-based G?+?C content was 65.5%. The average nucleotide identity and digital DNA-DNA hybridization values between strains EP178T and the closest type strain, P. oryzihabitans DSM 6835T, were 92.6% and 52.2%, respectively. Various genes associated with plant-growth promoting mechanisms were annotated from genome sequences. Based on the phenotypic, genomic, phylogeny and chemotaxonomic data, strain EP178T represents a new species of the genus Pseudomonas, for which the name Pseudomonas flavocrustae sp. nov. was proposed. The type strain is EP178T (=?CBMAI 2609T?=?ICMP 24844T?=?MUM 23.01T).

Publication year: 2024

Types of publication: Journal article

Unit: Embrapa Environment

Keywords: Bactéria, Endophytes, Estimulante de Crescimento Vegetal, Growth promotion, Maracujá, Passiflora incarnata, Passifloraceae, Pseudomonas sp