Identification of QTLs in the 'Araguaia' (Oryza sativa subsp. japonica) x 'Maninjau' (Oryza sativa subsp. indica) rice population.
Identification of QTLs in the 'Araguaia' (Oryza sativa subsp. japonica) x 'Maninjau' (Oryza sativa subsp. indica) rice population.
Author(s): PRADO, J. F. F. dos S.; CORDEIRO, A. C. C.; COELHO, A. S. G.; VALDISSER, P. A. M. R.; VIANELLO, R. P.; BRONDANI, C.
Summary: ABSTRACT - The objective of this work was to identify quantitative trait loci (QTLs) associated with the traits grain yield, plant height, and flowering, as well as superior inbred lines resulting from the intersubspecific cross between 'Araguaia' (Oryza sativa subsp. japonica) and 'Maninjau' (Oryza sativa subsp. indica) rice population. A population consisting of 234 recombinant inbred lines (RILs) was assessed in two environments and genotyped using single nucleotide polymorphisms (SNPs) and SilicoDArT markers. Twenty-two QTLs accounting for phenotypic variation ranging from 3.94% to 35.36%, were identified as significant, as follows: six for grain yield, five for flowering, and eleven for plant height. New QTLs were consistently identified for height and flowering traits with the SNP marker 12_22887040, in both environments, and highlighted for assisted selection of early rice varieties. In both environments, the RIL 1572 with the greatest productivity (6,581 kg ha-1), precocity of 70 days to flowering, and the lowest plant height (90 cm) is highly recommended for integration into crosses with elite materials from the rice breeding program. RESUMO - O objetivo deste trabalho foi identificar QTLs (quantitative trait loci) associados às características rendimento de grãos, altura de planta e florescimento, assim como linhagens superiores, na população oriunda do cruzamento inter-subespecífico 'Araguaia' (Oryza sativa subsp. japonica) x 'Maninjau' (Oryza sativa subsp. indica). Uma população composta por 234 linhagens puras recombinantes (RILs) foi avaliada em dois ambientes e genotipada por marcadores de polimorfismo de nucleotídeo único (SNPs – single nucleotide polymorphisms) e SilicoDArTs. Identificaram-se 22 QTLs com variação fenotípica explicada de 3,94% a 35,36%, que foram significativos, conforme a seguir: seis quanto à produtividade de grãos, cinco quanto ao florescimento e onze quanto à altura de planta. Foram encontrados QTLs inéditos quanto à altura de planta e ao florescimento. O marcador SNP 12_22887040 identificado nos dois ambientes é indicado para a seleção assistida de variedades de arroz mais precoces. Nos dois ambientes, a RIL 1572 – com maior produtividade (6,581 kg ha-1), precocidade de 70 dias até o florescimento e menor altura de planta (90 cm) – é altamente recomendada para a integração em cruzamentos com materiais-elite do programa de melhoramento de arroz.
Publication year: 2024
Types of publication: Journal article
Unit: Embrapa Roraima
Observation
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