Author(s): NEVES, M. R. M. das; ALVES, F. S. F.

Summary: Resumo: A coleção e manutenção de banco de germoplasma de material biológico e microorganismos causadores de problemas de caprinos e ovinos é essencial para estudos biomoleculares. A Corynebacterium pseudotuberculosis é o agente etiológico da Linfadenite Caseosa (LC) em pequenos ruminantes, enfermidade responsável por perdas econômicas graves nestas espécies no Nordeste do Brasil. O presente estudo teve como objetivo coletar material purulento em 55 animais de diferentes raças e idades. Na remoção do material purulento foi realizada abertura do abcesso e a coleta do pus. As amostras foram identificadas, acondicionadas em isopor com gelo e enviadas ao Laboratório de Bacteriologia da Embrapa Caprinos. No laboratório foram processadas para isolamento e identificação do agente, sendo mantido à -20°C. O plaqueamento foi feito em meio de cultura Agar sangue. Das 55 amostras examinadas, a C. pseudotuberculosis estava presente em todas, onde 46 (83,63%) eram de caprinos e nove (16,37%) de ovinos. As analises microbiológicas apresentaram cocobacilo Grampositivo e nos testes bioquímicos, catalase e uréia positiva. Nos açúcares manose, lactose, maltose, galactose, dextrose, sacarose, trealose, sorbitol, glicose, os microrganismos isolados foram divididos em 2 grupos, no qual fermentaram 17 (36,96%) caprinos e 2 (22,22%) ovinos. Abstract: The germoplasm bank collection and maintenance of biological material and microorganisms causing problems of goat and sheep is essential for biomolecular studies. The agent of Caseous lymphadenitis in small ruminants is Corynebacterium pseudotuberculosis, a illness that causing serious economical losses in Northeast of Brazil. The present study was to collect suppurating material from 55 different animals, breeding and age. To remove the suppurating material the abscess was opened, samples collected, identified and stored in a box with ice and sent to bacteriology laboratory at Embrapa Goat Center. The samples was processed for isolation and identification of the agent. All the suppurating material was stored and conserved -20°C. Blood agar was used to streaked the samples. All the 55 samples, C. pseudotuberculosis was presented and 46 (83.63%) were goat and nine (16.37%) sheep species. Microbiological analysis presented Gram-positive cocobacilus and the biochemical tests: catalase and urease positive. In sugars results from manose, lactose, maltose, galactose, dextrose, sacharose, trealose, sorbitol, glucose, the isolated microorganisms was divided in two groups, which they resulted in positive from 17 (36.96%) goat and 2 (22.22%) sheep.

Publication year: 2006

Types of publication: Paper in annals and proceedings

Unit: Embrapa Goats & Sheep

Keywords: Análise Microbiológica, Banco de Germoplasma, Biochemistry analysis, Caprino, Caseous lymphadenitis, Corynebacterium Pseudotuberculosis, Germoplasm bank, Goat diseases, Goats, Linfadenite Caseosa, Microorganismo, Ovino, Sheep diseases