Analysis of gene expression of in vitro produced bovine blastocyst cultured for 72 hours post-vitrification.
Analysis of gene expression of in vitro produced bovine blastocyst cultured for 72 hours post-vitrification.
Author(s): BOITÉ, M. C.; CAMARGO, L. S. de A.; MARTINS, M. F.; SERAPIÃO, R. V.; WOHLRES-VIANA, S.; VIANA, J. H. M.; SA, W. F. de; NOGUEIRA, L. A. G.
Summary: In vitro produced (IVP) embryos frequently show low survival rates after cryopreservation. Among the available methods, vitrification has generally shown better results with IVP embryos. However, little is known about vitrification effects on genes expression. The aim of this study was to evaluate the expression of aquaporins encoding genes for (AQP) 3, AQP11 and ATPase ?1 in vitrified/warming embryos cultured in vitro for 72h. AQPs are channels that facilitate water transportation across membranes, whereas ATPase ?1 is a Na/K pump subunit. Both AQPs and Na/K pump may be involved in blastocoel expansion after cryopreservation
Publication year: 2008
Types of publication: Abstract in annals or event proceedings
Unit: Embrapa Dairy Cattle
Keywords: Bovino, Expressão genética
Observation
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