Purificação de IgG de codorna a partir de soro e gema de ovos.
Purificação de IgG de codorna a partir de soro e gema de ovos.
Author(s): OLIVEIRA, D. D.; RESENDE, J. S.; FLATSCHAR, A. V. F.; ABREU, J. T.; FLATSCHART, R. B.; MARTINS, N. R. S.
Summary: The purification of Japanese quail IgG from serum was performed using caprilic acid and ammonium sulfate, and from egg yolk using PEG-6000 and ethanol. After confirming the purification and the concentration of IgG, the yolk samples had twice the amount of protein compared to serum samples. The IgG extracts were analyzed by SDS-PAGE and western blot showing similar results as the ones of chicken IgG used as the standard. So, these methodologies can be used for purifying quail serum or egg yolk IgG, which would enable the development of diagnostic assays.
Publication year: 2010
Types of publication: Journal article
Unit: Embrapa Maize & Sorghum
Keywords: Imunoglobulina, Soro
Observation
Some of Embrapa's publications are published as ePub files. To read them, use or download one of the following free software options to your computer or mobile device. Android: Google Play Books; IOS: iBooks; Windows and Linux: Calibre.
Access other publications
Access the Agricultural Research Database (BDPA) to consult Embrapa's full library collection and records.
Visit Embrapa Bookstore to purchase books and other publications sold by Embrapa.