Autoria: NASCIMENTO, C. S. do; MACHADO, M. A.; GUIMARÃES, S. E. F.; GUIMARAES, M. F. M.; PEIXOTO, J. O.; FURLONG, J.; PRATA, M. C. de A.; VERNEQUE, R. da S.; TEODORO, R. L.; LOPES, P. S.

Resumo: The bovine tick Rhipicephalus (Boophilus) microplus causes major losses in cattle production systems in tropical regions. Bos indicus breeds are more resistant to ticks than B. taurus breeds. Resistance genes could be an alternative to control this parasite. We examined the pattern of gene expression of three calcium-binding-protein genes: translationally controlled tumor protein 1 (TPT1), allergen Bos d3 (S100A7), calcium channel protein transient receptor potential vanilloid 6 (TRPV6), and the cysteine proteinase inhibitor gene (CST6). These genes were selected from cDNA libraries prepared from skin biopsies taken from resistant and susceptible Gyr x Holstein F? animals. These biopsies were also used to study the expression level of these genes through real-time PCR analysis. The relative expression levels of the S100A7, TPT1, TRPV6, and CST6 genes were 2.01 ± 0.6, 1.32 ± 0.9, 1.53 ± 1.2, and 2.03 ± 0.7 times higher in the susceptible group, respectively. Skin lesion tissue from the susceptible animals showed significantly more mRNA transcripts of these genes in comparison with the resistant animals (P = 0.001). However, this hypersensitivity does not seem to protect the susceptible animals against tick infestation.

Ano de publicação: 2010

Tipo de publicação: Artigo de periódico

Unidade: Embrapa Gado de Leite

Palavras-chave: CST6, QRT-PCR, S100A7, TPT1, TRPV6, Tick resistance