Genetic characterization of an elite coffee germplasm assessed by gSSR and EST-SSR markers.
Genetic characterization of an elite coffee germplasm assessed by gSSR and EST-SSR markers.
Autoria: MISSIO, R. F.; CAIXETA, E. T.; ZAMBOLIM, E. M.; PENA, G. F.; ZAMBOLIM, L.; DIAS, L. A. S.; SAKIYAMA, N. S.
Resumo: Coffee is one of the main agrifood commodities traded worldwide. In 2009, coffee accounted for 6.1% of the value of Brazilian agricultural production, generating a revenue of US$6 billion. Despite the importance of coffee production in Brazil, it is supported by a narrow genetic base, with few accessions. Molecular differentiation and diversity of a coffee breeding program were assessed with gSSR and EST-SSR markers. The study comprised 24 coffee accessions according to their genetic origin: arabica accessions (six traditional genotypes of C. arabica), resistant arabica (six leaf rust-resistant C. arabica genotypes with introgression of Híbrido de Timor), robusta (five C. canephora genotypes), Híbrido de Timor (three C. arabica x C. canephora), triploids (three C. arabica x C. racemosa), and racemosa (one C. racemosa). Allele and polymorphism analysis, AMOVA, the Student t-test, Jaccard?s dissimilarity coefficient, cluster analysis, correlation of genetic distances, and discriminant analysis, were performed. EST-SSR markers gave 25 exclusive alleles per genetic group, while gSSR showed 47, which will be useful for differentiating accessions and for fingerprinting varieties. The gSSR markers detected a higher percentage of polymorphism among (35% higher on average) and within (42.9% higher on average) the genetic groups, compared to EST-SSR markers. The highest percentage of polymorphism within the genetic groups was found with gSSR markers for robusta (89.2%) and for resistant arabica (39.5%). It was possible to differentiate all genotypes including the arabica-related accessions. Nevertheless, combined use of gSSR and EST-SSR markers is recommended for coffee molecular characterization, because EST-SSRs can provide complementary information.
Ano de publicação: 2011
Tipo de publicação: Artigo de periódico
Unidade: Embrapa Café
Palavras-chave: Coffea, Discriminant analysis, Genetic diversity, Microsatellite marker
Observações
1 - Por padrão são exibidas publicações dos últimos 20 anos. Para encontrar publicações mais antigas, configure o filtro ano de publicação, colocando o ano a partir do qual você deseja encontrar publicações. O filtro está na coluna da esquerda na busca acima.
2 - Para ler algumas publicações da Embrapa (apenas as que estão em formato ePub), é necessário ter, no celular ou computador, um desses softwares gratuitos. Sistemas Android: Google Play Livros; IOS: iBooks; Windows e Linux: software Calibre.
Acesse outras publicações
Acesse a Base de Dados da Pesquisa Agropecuária (BDPA) para consultar o acervo completo das bibliotecas da Embrapa.