In tropical regions, bovine infestation by ticks causes a drastic reduction in milk and meat yield, leading in some cases, to the death of animals. Chemicals have been typically used to combat ticks, however, this strategy leads to the contamination of milk and meat products in addition to the environment. The genetic variability between Bos taurus and Bos indicus breeds for tick resistance and the currently available molecular genetics tools make it possible to use this information in breeding programs in order to obtain more productive and adapted animals, by means of marker-assisted selection or transgene. A population of crossbred animals (Holstein x Gir) was used to identify genes related to resistance to ticks. Two cDNA libraries were generated from animal skin of infected resistant and susceptible animals to bovine tick. More than 4,000 clones from the libraries were sequenced and the patterns of differential gene expression between the libraries sequences derived from resistant and susceptible animals were studied. The expression of biological candidate genes linked to the production of cytokines was studied using the real-time PCR technique. By means of the microarray technique, the profiles of differential gene expression between cDNA samples from tick resistant and susceptible animals were analyzed for more than 23,000 genes. At the end, this project allowed a better understanding of the metabolic pathways involved in the mechanisms underlying bovine tick resistance, through the identification of dozens of candidate genes. New experiments should be conducted to validate the action of these genes.