This project involved five Institutional Collections of Microorganisms (CIs) and five Working Collections (CTs) associated with three of the CIs. Each CI was dedicated to a specific Institutional Collection, and was based in an Embrapa's Decentralized Unit, as follows: Collection of Microorganisms of Agroindustrial Interest (Embrapa Grape & Wine), Collection of Microorganisms of Interest to Agroindustry and Livestock (Embrapa Dairy Cattle), Collection of Microorganisms of Interest for Swine and Poultry (Embrapa Swine & Poultry), Collection of Multifunctional and Phytopathogenic Microorganisms (Embrapa Rice & Beans), and Collection of Multifunctional and Phytopathogenic Microorganisms (Embrapa Maize & Sorghum). The participating CTs were: Collection of Microorganisms of Interest to the Food and Agroenergy Industry (Embrapa Food Technology), Collection of Microorganisms of Interest to the Tropical Agroindustry (Embrapa Tropical Agroindustry), Collection of Pathogenic Microorganisms to Goats and Sheep (Embrapa Goats & Sheep), Collection of Phytopathogenic Microorganisms of Forest Species (Embrapa Forestry) and Collection of Microorganisms of Quarantine Importance (Embrapa Genetic Resources and Biotechnology). In total, ten Embrapa´s Decentralized Units were involved. Both CIs and CTs successfully carried out activities to collect, isolate, identify, characterize, prospect and store microorganisms, and collection documentation. Such documentation implies in the registration of strains and their respective information in Embrapa’s AleloMicro system. In addition, while conducting the project, quality requirements were implemented in the Collections, foreseen and monitored in another Embrapa´s project. All the carried out activities were essential for the enrichment and continuity of the Collections. The continuity of the Collections is of great importance for the preservation of biodiversity and for the development of research, products and technologies. The microorganisms in the Collections are preserved by long-term methods, according to the type of microorganism, such as freezing, deep freezing, lyophilization, drying on filter paper, Castellani method and preservation in oil. Many of the preserved microorganisms were characterized by different methods in the respective SIs, generating results published in scientific articles, Master's and Doctoral dissertations and theses, technical articles and complete articles and abstracts in conference proceedings. Within the Collections or the SIs, microorganisms were also exchanged for research purposes or use in products. Some microorganisms present in the Collections are used in plant genetic improvement programs for the selection of resistant varieties or exploited to obtain bioinputs. As part of the project, laboratory tests were carried out to prospect abiofungicide product.