The use of essential oils is a very promising alternative in the control of monogenous worms in tambaqui (Colossoma macropomum), however there are few studies on the antihelmintic activity of oils in these ectoparasites, considering the great diversity of bioactive substances they have. This study aims to establish strategies in vitro and in vivo (therapeutic baths) for monogenesis control in tambaqui using essential oil of Cymbopogom citratus (lemongrass or lemongrass), Lippia grata (Rosemary-do-mato) and Alpinia zerumbet (Ginger shell or False-cardamom), after carrying out the tolerance tests. The results of this work aim to contribute to scientific and technological development and indicate the best strategy for controlling these ectoparasites using essential oils.