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Production of bovine transgenic embryos by microinjection of a lentiviral vector in mature ovocytes. Author(s): OTERO, R.; HERNÁNDEZ, D.; CAMARGO, L. S. de A. Abstract Objective: To produce bovine transgenic embryos by microinjection of a lentiviral vector with the eGFP gene as a marker. Methods: Four treatments were designed: T1=Control: fertilized in vitr... ... Repository: BDPA Publication year: 2018
Unlocking nature’s shield: the promising potential of CRISPRa in amplifying antimicrobial peptide expression in common bean (Phaseolus vulgaris L.). Author(s): MAXIMIANO, M. R.; SOUSA, L. J. de; FEITOSA, G. C.; LOPES, M. E. M.; ORTEGA, B.; MADEIRO, R. dos S.; TÁVORA, F. T. P. K.; PEREIRA, B. M.; OLIVEIRA NETO, O. B. de; ULHÔA, C. J.; BRASILEIRO, A. C. M.; ARAGÃO, F. J. L.; REIS, A. M. dos; FRANCO, O. L. This study proposes using the CRISPR transcriptional activation strategy to modulate the expression of genes encoding defense proteins and antimicrobial peptides (AMPs) in Phaseolus vulgaris. Three ge... ... Repository: BDPA Publication year: 2025

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