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Chemically induced enucleation of activated bovine oocytes: chromatin and microtubule organization and production of viable cytoplasts. Author(s): SARAIVA, N. Z.; OLIVEIRA, C. S.; LEAL, C. L. V.; CALLADO, M. del; VANTINI, R.; MONTEIRO, F. M.; NICIURA, S. C. M.; GARCIA, J. M. As the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these t... ... Repository: BDPA Publication year: 2015
Effects of demecolcine on microtubule composition and chemically assisted enucleation of bovine oocytes. Author(s): SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; OLIVEIRA, J. C.; GARCIA, J. M. The developmental competence of enucleated oocytes is a key factor that determines the overall success of animal cloning. Enucleation is an invasive procedure in traditional nuclear transfer (NT). The... ... Repository: BDPA Publication year: 2008
Protocol optimization and evaluation of maturation promoting factor and mitogen-activated protein kina sea ctivities in bovine cytoplasts obtained by chemical enucleation techniques. Author(s): SARAIVA, N. Z.; OLIVEIRA, C. S.; COLLADO, M. del; LIMA, M. R. de; VANTINI, R.; MONTEIRO, F. M.; NICIURA, S. C. M.; GARCIA, J. M. Chemical enucleation using microtubule-depolymerizing drugs is an attractive procedure to simplify the enucleation process in nuclear transfer. Repository: BDPA Publication year: 2013
Feasibility of In Vivo semen collection and description of the morphology and ultrastructure of the spermatozoa of Arapaima gigas (Schinz, 1822). Author(s): TORATI, L. S.; LOPES, J. T.; LIMA, A. F.; PUVANENDRAN, V.; BATLOUNI, S. R.; KIRSCHNIK, L. N. G. The pirarucu, Arapaima gigas, is an emblematic endangered Amazon osteoglossid with high potential for aquaculture development. In this study, we report the in vivo collection of semen from pirarucu fo... ... Repository: BDPA Publication year: 2024
A differential phenotypic expression of a divergent spindle mutation in interspecific Brachiaria hybrids. Author(s): FELISMINO, M. F.; PAGLIARINI, M. S.; VALLE, C. B. do Several mutations are known to alter the normal progression of meiosis and can be correlated with defects in microtubule distribution. The dv mutation affects the spindle organization and chromosomes... ... Repository: BDPA Publication year: 2008
A Non-synonymous point mutation in a WD-40 domain repeat of EML5 leads to decreased bovine sperm quality and fertility. Author(s): NOGUEIRA, E.; TIRPÁK, F.; HAMILTON, L.; ZIGO, M.; KERNS, K.; SUTOVSKY, M.; KIM, J.; VOLKMANN, D.; JOVINE, L.; TAYLOR, J. F.; SCHNABEL, R. D.; SUTOVSKY, P. This study is part of a concerted effort to identify and phenotype rare, deleterious mutations that adversely affect sperm quality, or convey high developmental and fertility potential to embryos and... ... Repository: BDPA Publication year: 2022

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