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Identification of a novel reference gene for apple transcriptional profiling under postharvest conditions. Author(s): QUECINI, V.; GIRARDI, C. L. Repository: BDPA Publication year: 2015
Reference gene selection for gene expression studies in apple. Author(s): OLIVEIRA, P. R. D. de; REVERS, L. F. Apple (Malus x domestica Borkh.) is the most important deciduous tree fruit crop grown around the world, and also in the Southern of Brazil. Researches on apple genetic breeding include disease resist... ... Repository: BDPA Publication year: 2011
Validation of reference housekeeping genes for gene expression studies in western corn rootworm (Diabrotica virgifera virgifera). Author(s): VALICENTE, F. H. Quantitative Real-time PCR (qRT-PCR) is a powerful technique to investigate comparative gene expression. In general, normalization of results using a highly stable housekeeping gene (HKG) as an intern... ... Repository: BDPA Publication year: 2014
Reference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions. Author(s): NEUMAIER, N.; MARCELINO-GUIMARÃES, F. C.; FARIAS, J. R. B.; OLIVEIRA, M. C. N. de; OLIVEIRA, A. C. B. de; EMYGDIO, B. M.; NEPOMUCENO, A. L. Quantitative real-time polymerase chain reaction (RT-qPCR) is a powerful tool used to measure gene expression. However, because of its high sensitivity, the method is strongly influenced by the qualit... ... Repository: BDPA Publication year: 2014
Selection of Reference Genes for RT-qPCR Studies in Different Organs of Rice Cultivar BRS AG Submitted to Recurrent Saline Stress. Author(s): MAGALHAES JUNIOR, A. M. de Quantitative real-time polymerase chain reactions (RT-qPCR) have become one of the most widely used methods for analyzing gene expression, provided suitable reference genes are available to normalize... ... Repository: BDPA Publication year: 2021
An improved method for RNA extraction from common bean seeds and validation of reference genes for qPCR. Author(s): BASSINELLO, P. Z.; BRONDANI, C.; VIANELLO, R. P. An RNA extraction method with high integrity and purity as well as the selection of adequate reference genes are prerequisites for gene expression analysis. For common bean seeds, there is no well-def... ... Repository: BDPA Publication year: 2017
The use of microRNAs as universal reference genes in quantitative PCR. Author(s): MARCELINO, F. C.; NEPOMUCENO, A. L.; ABDELNOOR, R. V. Reverse transcription quantitative real-time PCR (RT-qPCR) is a robust and widely applied technique used to investigate gene expression. However, for correct analysis and interpretation of results, th... ... Repository: BDPA Publication year: 2010
Reference genes for transcriptional analysis of flowering and fruit ripening stages in apple (Malus 3 domestica Borkh.). Author(s): OLIVEIRA, P. R. D. de; REVERS, L. F. Apple (Malus 9 domestica Borkh.) is the most important deciduous tree fruit crop grown around the world. Comparisons of gene expression profiles from different tissues, conditions or cultivars are val... ... Repository: BDPA Publication year: 2014
Identification of reference genes for expression analysis by real-time quantitative PCR in drought-stressed soybean. Author(s): ABDELNOOR, R. V.; OLIVEIRA, M. C. N. de; NEPOMUCENO, A. L.; MARCELINO-GUIMARÃES, F. C. The objective of this work was to validate, by quantitative PCR in real time (RT-qPCR), genes to be used as reference in studies of gene expression in soybean in drought-stressed trials. Four genes co... ... Repository: BDPA Publication year: 2011
Evaluation of reference genes for real-time PCR studies of Brazilian Somalis sheep infected by gastrointestinal nematodes. Author(s): SIDER, L. H.; VIEIRA, L. da S. Abstract: Precise normalization with reference genes is necessary, in order to obtain reliable relative expression data in response to gastrointestinal nematode infection. By using sheep from temperat... ... Repository: BDPA Publication year: 2010

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