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Author(s): STORCH, T. T.; PEGORARO, C.; FINATTO, T.; QUECINI, V.; ROMBALDI, C. V.; GIRARDI, C. L.

Repository: BDPA     Publication year: 2015

Author(s): PERINI, P.; PASQUALI, G.; MÁRGIS-PINHEIRO, M.; OLIVEIRA, P. R. D. de; REVERS, L. F.

Apple (Malus x domestica Borkh.) is the most important deciduous tree fruit crop grown around the world, and also in the Southern of Brazil. Researches on apple genetic breeding include disease resist... ...

Repository: BDPA     Publication year: 2011

Author(s): RODRIGUES, T. B.; KHAJURIA, C.; WANG, H.; MATZ, N.; CARDOSO, D. C.; VALICENTE, F. H.; ZHOU, X.; SIEGFRIED, B.

Quantitative Real-time PCR (qRT-PCR) is a powerful technique to investigate comparative gene expression. In general, normalization of results using a highly stable housekeeping gene (HKG) as an intern... ...

Repository: BDPA     Publication year: 2014

Author(s): NAKAYAMA, T. J.; RODRIGUES, F. A.; NEUMAIER, N.; MARCELINO-GUIMARÃES, F. C.; FARIAS, J. R. B.; OLIVEIRA, M. C. N. de; BORÉM, A.; OLIVEIRA, A. C. B. de; EMYGDIO, B. M.; NEPOMUCENO, A. L.

Quantitative real-time polymerase chain reaction (RT-qPCR) is a powerful tool used to measure gene expression. However, because of its high sensitivity, the method is strongly influenced by the qualit... ...

Repository: BDPA     Publication year: 2014

Author(s): ROSSATTO, T.; AULER, P. A.; AMARAL, M. N.; MILECH, C.; MAGALHAES JUNIOR, A. M. de; BRAGA, E. J. B.

Quantitative real-time polymerase chain reactions (RT-qPCR) have become one of the most widely used methods for analyzing gene expression, provided suitable reference genes are available to normalize... ...

Repository: BDPA     Publication year: 2021

Author(s): PEREIRA, W. J.; BASSINELLO, P. Z.; BRONDANI, C.; VIANELLO, R. P.

An RNA extraction method with high integrity and purity as well as the selection of adequate reference genes are prerequisites for gene expression analysis. For common bean seeds, there is no well-def... ...

Repository: BDPA     Publication year: 2017

Author(s): KULCHESKI, F. R.; MARCELINO, F. C.; NEPOMUCENO, A. L.; ABDELNOOR, R. V.; MARGIS, R.

Reverse transcription quantitative real-time PCR (RT-qPCR) is a robust and widely applied technique used to investigate gene expression. However, for correct analysis and interpretation of results, th... ...

Repository: BDPA     Publication year: 2010

Author(s): PERINI, P.; PASQUALI, G.; MARGIS-PINHEIRO, M.; OLIVEIRA, P. R. D. de; REVERS, L. F.

Apple (Malus 9 domestica Borkh.) is the most important deciduous tree fruit crop grown around the world. Comparisons of gene expression profiles from different tissues, conditions or cultivars are val... ...

Repository: BDPA     Publication year: 2014

Author(s): STOLF-MOREIRA, R.; LEMOS, E. G. de M.; ABDELNOOR, R. V.; BENEVENTI, M. A.; ROLLA, A. A. P.; PEREIRA, S. dos S.; OLIVEIRA, M. C. N. de; NEPOMUCENO, A. L.; MARCELINO-GUIMARÃES, F. C.

The objective of this work was to validate, by quantitative PCR in real time (RT-qPCR), genes to be used as reference in studies of gene expression in soybean in drought-stressed trials. Four genes co... ...

Repository: BDPA     Publication year: 2011

Author(s): ZAROS, L. G.; COUTINHO, L. L.; SIDER, L. H.; MEDEIROS, H. R. de; NEVES, M. R. M. das; BENVENUTI, C. L.; NAVARRO, A. M. do C.; VIEIRA, L. da S.

Abstract: Precise normalization with reference genes is necessary, in order to obtain reliable relative expression data in response to gastrointestinal nematode infection. By using sheep from temperat... ...

Repository: BDPA     Publication year: 2010

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