Reproductive biotechnologies, particularly in vitro embryo production, can be useful to improve the milk productivity in Brazil by accelerating the genetic gain and reducing the intervals between generations. Genomic selection associated with reproductive biotechnology may be applied at the embryonic stage to promote the selection of embryos with desirable genetic traits and disposal of those with undesirable traits; consequently, maintenance costs of recipient cows would be reduced and efforts could be focused on embryos that will generate animals with higher dairy performance. Those analysis can be made on embryonic cells obtained by biopsies. Furthermore, analysis of those cells may be useful to inform the viability of the embryo, allowing the identification of those with the greatest development potential and hence greater efficiency. Therefore, the aim is to evaluate the application of biopsy of in vitro-produced embryos for genotyping, as well as identify and assess chromosomal changes that may be associated with lower embryonic development. Embryos are produced in the laboratories of Embrapa Dairy Cattle and submitted to biopsies in order to identify the effects of the technique on their viability through quantification of apoptotic cells, number of total cell counts and assessment of blastocyst hatching. To prove the safety of the technique, embryos are transferred to recipients and pregnancies are assisted until the birth. Data of the embryos and of the respective calves are compared in order to evaluate the genotyping concordance between them. It is expected to contribute to increased reliability and widespread use of embryo biopsy followed by genotyping favoring the selection of embryos with desirable genetic traits and more viability for adoption in the official breeding programs or in commercial herds.