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The technology for gene editing based on the CRISPR / Cas9 system (Clustered Regularly-Interspaced Short Palindromic Repeats associated to Cas9 endonuclease) consists of an endonuclease (Cas9) guided by RNA (guide RNA - sgRNA), which is capable of editing the genome with accuracy. This system enables the inactivation of genes through cleavage followed by repairing the strands by non-homologous end joining (NHEJ) leading to the knockout of the expression, which results extremely useful to prevent Status: Completed Start date: Tue Jan 01 00:00:00 GMT-03:00 2019 |
The technology for gene editing based on the CRISPR / Cas9 system (Clustered Regularly-Interspaced Short Palindromic Repeats associated to Cas9 endonuclease) consists of an endonuclease (Cas9) guided by RNA (guide RNA - sgRNA), which is capable of editing the genome with accuracy. This system enables the inactivation of genes through cleavage followed by repairing the strands by non-homologous end joining (NHEJ) leading to the knockout of the expression, which results extremely useful to prevent Status: Completed Start date: Mon Jan 01 00:00:00 GMT-03:00 2018 |
The proposal presents an innovative solution, which is to validate candidate drought tolerance genes through genetic transformation (cisgeny) for the development of a banana variety with efficient use of water and a case study with CRISPR/cas9 via gene knock-out PDS (phytoene desaturase) as a model for the species. Status: Completed Start date: Fri Jan 01 00:00:00 GMT-03:00 2021 |
Proteins found in cow's milk are one of the main sources of childhood allergies and can affect up to 7% of children. A more common allergen is beta-lactoglobulin (BGL), which makes up more than 50% of whey protein and is absent in human milk. Hypoallergenic cow's milk can be obtained by treating the milk, but this can result in residual antigenicity and alter the taste of the milk. Additionally, prices are 10-15x higher than conventional milk. The bovine BGL gene is located on chromosome 11 and Status: Completed Start date: Tue Jan 01 00:00:00 GMT-03:00 2019 |