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Clonagem e análise de expressão de candidatos a genes de referência em tambaqui (Colossoma macropomum). Author(s): NASCIMENTO, A. R. do; O'SULLIVAN, F. A.; SILVA, G. F. da Neste trabalho, os genes 18S, beta-actina, gapdh e ef1alfa do tambaqui foram clonados e sequenciados, visando à obtenção e validação de genes de referência, candidatos aos estudos genômicos e de fisio... ... Repository: BDPA Publication year: 2016
Selection of reliable reference genes for quantitative real-time polymerase chain reaction studies in maize grains. Author(s): GALLI, V.; MESSIAS, R. da S.; SILVA, S. D. dos A. e; ROMBALDI, C. V. Repository: BDPA Publication year: 2013
Reference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions. Author(s): NAKAYAMA, T. J.; RODRIGUES, F. A.; NEUMAIER, N.; MARCELINO-GUIMARÃES, F. C.; FARIAS, J. R. B.; OLIVEIRA, M. C. N. de; BORÉM, A.; OLIVEIRA, A. C. B. de; EMYGDIO, B. M.; NEPOMUCENO, A. L. Quantitative real-time polymerase chain reaction (RT-qPCR) is a powerful tool used to measure gene expression. However, because of its high sensitivity, the method is strongly influenced by the qualit... ... Repository: BDPA Publication year: 2014
An improved method for RNA extraction from common bean seeds and validation of reference genes for qPCR. Author(s): PEREIRA, W. J.; BASSINELLO, P. Z.; BRONDANI, C.; VIANELLO, R. P. An RNA extraction method with high integrity and purity as well as the selection of adequate reference genes are prerequisites for gene expression analysis. For common bean seeds, there is no well-def... ... Repository: BDPA Publication year: 2017
Development of reference genes for RT-qPCR analysis of gene expression in Pleurotus pulmonarius for biotechnological applications. Author(s): GOMES, T. G.; FONSECA, F. C. de A.; ALVES, G. S. C.; SIQUEIRA, F. G. de; MILLER, R. N. G. Jatropha curcas is an oilseed crop with biorefinery applications. Whilst cake generated following oil extraction offers potential as a protein source for animal feed, inactivation of toxic phorbol est... ... Repository: BDPA Publication year: 2023
Selection of Reference Genes for RT-qPCR Studies in Different Organs of Rice Cultivar BRS AG Submitted to Recurrent Saline Stress. Author(s): ROSSATTO, T.; AULER, P. A.; AMARAL, M. N.; MILECH, C.; MAGALHAES JUNIOR, A. M. de; BRAGA, E. J. B. Quantitative real-time polymerase chain reactions (RT-qPCR) have become one of the most widely used methods for analyzing gene expression, provided suitable reference genes are available to normalize... ... Repository: BDPA Publication year: 2021
Selection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and drought. Author(s): BEVITORI, R.; OLIVEIRA, M. B.; GROSSI-DE-SÁ, M. F.; LANNA, A. C.; SILVEIRA, R. D. da; PETROFEZA, S. Drought and rice blast disease caused by Magnaporthe oryzae are two of the most serious threats to global rice production. To explore the mechanisms underlying gene expression induced in rice by stres... ... Repository: BDPA Publication year: 2014
Validation of reference housekeeping genes for gene expression studies in western corn rootworm (Diabrotica virgifera virgifera). Author(s): RODRIGUES, T. B.; KHAJURIA, C.; WANG, H.; MATZ, N.; CARDOSO, D. C.; VALICENTE, F. H.; ZHOU, X.; SIEGFRIED, B. Quantitative Real-time PCR (qRT-PCR) is a powerful technique to investigate comparative gene expression. In general, normalization of results using a highly stable housekeeping gene (HKG) as an intern... ... Repository: BDPA Publication year: 2014
Selection of reference genes for expression study in pulp and seeds of Theobroma grandiflorum (Willd. ex Spreng.) Schum. Author(s): SANTOS, L. F. dos; SILVA, R. J. S.; AMARAL, D. O. J. do; PAULA, M. F. B. de; FALCÃO, L. L.; LEGAVRE, T.; ALVES, R. M.; MARCELLINO, L. H.; MICHELI, F. Cupuassu (Theobroma grandiflorum [Willd. ex Spreng.] Schum) is a species of high economic importance in Brazil with great potential at international level due to the multiple uses of both its seeds an... ... Repository: BDPA Publication year: 2016
Seleção de genes endógenos para análise de qRT-PCR em sementes de café. Author(s): SCHENK, J. C. M.; CARDOSO, D. C.; MALUF, M. P.; PADILHA, L. A técnica quantitativa de PCR em tempo real, qRT-PCR, permite analisar a expressão de genes alvo em diferentes condições experimentais. No entanto, a análise precisa dos resultados gerados pela qRT-PC... ... Repository: BDPA Publication year: 2013

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