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Author(s): SANTOS, F. C.; ROSA, S. D. V. F. da; GROTEWOLD, E.; PADILHA, L.

Coffee is one of the most important agricultural products in the world market. The mode of coffee processing, whether wet or dry, determines the flavor characteristic and also the seed quality. Qualit... ...

Repository: BDPA     Publication year: 2014

Author(s): PEREIRA, W. J.; BASSINELLO, P. Z.; BRONDANI, C.; VIANELLO, R. P.

An RNA extraction method with high integrity and purity as well as the selection of adequate reference genes are prerequisites for gene expression analysis. For common bean seeds, there is no well-def... ...

Repository: BDPA     Publication year: 2017

Author(s): FERREIRA, C. F.; GUTIERREZ, D. L.; KREUZE, J. F.; ISKRA-CARUANA, M. L.; CHABANNES, M.; BARBOSA, A. C. O.; SANTOS, T. A.; SILVA, A. G. S.; SANTOS, R. M. F.; AMORIM, E. P.; OLIVEIRA, S. A. S. de; JESUS, O. N. de

Plant DNA and RNA extraction methods are well established, with a wide range of protocols, depending on the purposes of each laboratory/research. Nowadays, quick, inexpensive and easy plant DNA and RN... ...

Repository: BDPA     Publication year: 2019

Author(s): INGLIS, P. W.; PAPPAS, M. de C. R.; RESENDE, L. V.; GRATTAPAGLIA, D.

Repository: BDPA     Publication year: 2018

Author(s): OLIVEIRA, J. C. de; SILVA, C. C. da; SOUZA, A. P. de; CAMPOS, T. de

The development of specific protocols for extraction of high-quality RNA are key advances in molecular techniques for elucidating biological processes and mechanisms. The forage peanut has significant... ...

Repository: BDPA     Publication year: 2020

Author(s): CORDEIRO, M. C. R.; FRAGOSO, R. da R.; SILVA, M. S.; BARROS, L. M. G.

Repository: BDPA     Publication year: 2010

Author(s): FONSECA, M. L. da S.; ANDRADE, E. C. de

Objetivo: O objetivo deste trabalho foi analisar a viabilidade ao longo do tempo de bactérias engenheiradas para produção de dsRNA mantidas em meio de cultura sólido, visando estabelecer o período máx... ...

Repository: BDPA     Publication year: 2021

Author(s): PROSDOCIMI, F.; BITTENCOURT, D. M. de C.; SILVA, F. R. D.; MOTTA, P. C.; RECH FILHO, E. L.

RNA-seq experiments conducted in 454 sequencers were carried out to produce 87.000 short-reads representing the transcriptome of two spiders'spinning glands. We produced sequences from (i) Actinopus s... ...

Repository: BDPA     Publication year: 2010

Author(s): OLIVEIRA, F. A. de; VIGNA, B. B. Z.; SILVA, C. C. da; FAVERO, A. P.; MATTA, F. de P.; AZEVEDO, A. L. S.; SOUZA, A. P. de

RNA sequencing (RNA-seq) is the most effective method for simultaneously predicting new transcripts and identifying differentially expressed genes among distinct tissues, genotypes, abiotic conditions... ...

Repository: BDPA     Publication year: 2020

Author(s): ITO, E. A.; KATAHIRA, I.; VICENTE, F. F. da R.; PEREIRA, L. F. P.; LOPES, F. M.

With the emergence of Next Generation Sequencing (NGS) technologies, a large volume of sequence data in particular de novo sequencing was rapidly produced at relatively low costs. In this context, com... ...

Repository: BDPA     Publication year: 2018

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