Among the many plagues which attack worldwide fruit culture, the fruit flies (Diptera: Tephritidae) stand out for being present in all continents, and in practically all types of environment, being thus great hinderers of worldwide fruit production. In the Amazonian region, 5 species are already known in Acre, 28 in Amazonas, 22 in Amapá. 20 in Pará, 5 in Rondônia, 13 in Roraima and 16 in Tocantins, belonging to the genera Anastrepha, Bactrocera and Ceratitis./nThe state of Pará presents great diversity and availability of fruits (mixed berries and cultivated fruits), a fact which contributes to the continuity and generation overlapping of different fruit fly species. Thusly, it becomes greatly important to invest in researches which aim to catalog their species, their host plants and natural enemies (parasitoids), in different fruit cultivation systems established throughout the region./nThis research project aims to determine fruit fly diversity and its natural enemies, as well as the infestation level in fruits from different cultivation systems in the county of Tomé-Açu (PA). For such, research actions will be implemented in three fruit cultivation systems (conventional, organic and Agro-forest System – SAF), in the hopes to identify in these environments the various species of fruit flies, their hosts and natural enemies by means of lab collection and processing of fruits.